High Performance Liquid Chromatography (HPLC)
High Performance Liquid Chromatography (HPLC) is an analytical technique which utilizes a compound’s intrinsic affinity for both a “mobile phase” (typically a buffered solvent) and a “stationary phase” (porous solid support with specialized coating). Essentially, a pump is used to provide a continuous flow of a solvent into which a dissolved sample is introduced. Once the sample is in the solvent flow, it travels through an analytical column. The compounds present in the sample mixture are then separated depending on their affinity to the coated particles in the column. After the components in the sample are separated, they may pass through an assortment of detectors. The detector response and the “retention time” (time it takes for a compound to pass from the injector to the detector) of the compound(s) of interest may then be compared to a reference material.
At EAG’s analytical labs, a number of chromatographic techniques may be used to customize High Performance Liquid Chromatography analysis for a specific sample matrix.
- Reversed Phase Chromatography (RP)
- Normal Phase Chromatography (NP)
- Ion Chromatography (IC)
- Gel Permeation / Size Exclusion Chromatography (GPC/SEC)
- Hydrophobic Interaction Chromatography (HILIC)
- Ion-Pairing Chromatography
- Chiral Chromatography
- Ion-Exchange/Ion Exclusion Chromatography
In addition, a number of detection techniques may be utilized to analyze for a specific type of compound.
- Spectrophotometric Detection (UV-Vis)
- Evaporative Light Scattering Detection (ELSD)
- Electrospray Ionization-Ion Trap Mass Spectrometry Detection (ESI-IT-MS)
- Atmospheric Pressure Chemical Ionization-Ion Trap Mass Spectrometry Detection (APCI-IT-MS)
- Refractive Index Detection (RI)
- Fluorescence Detection (FLD)
- Conductivity Detection