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Ultraviolet–Visible Spectroscopy (UV-Vis)

UV-Vis Spectroscopy is used to determine analyte concentration either at one time or often over a desired time period.  The technique measures the absorption of light across the ultraviolet and visible light wavelengths through a liquid sample.  Samples are dispensed into cuvette and placed in the path between a UV-Vis light and a detector.  According to Beer-Lambert’s law, with a constant light path length and known absorption coefficient  (dependent upon wavelength),  the concentration of a compound in question can be determined from the light absorbed by the sample at that wavelength.

Ideal Uses
  • Quantitation of analytes
  • Quality assurance and control
  • Maximum wavelength determination
Technical Specifications

Wavelength range 190-1100 nm

Strengths
  • Fast sample analysis
  • Suitable for a wide variety of analytes
  • Much simpler than chromatographic techniques
  • User-friendly interface
  • Little maintenance required
Limitations
  • Subject to fluctuations from stray light and temperature changes
  • Relatively low sensitivity
  • Other sample components may cause interferences
  • Not as specific as chromatography
  • Requires a relatively large sample volume, > 0.2 mL