Industries
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Pharmaceutical & Biopharmaceutical Development Services

EAG brings unparalleled expertise to the development and commercialization of small molecule drugs, biopharmaceuticals, antibody-drug conjugates (ADCs), drug-device combination…

EAG brings unparalleled expertise to the development and commercialization of small molecule drugs, biopharmaceuticals, antibody-drug conjugates (ADCs), drug-device combination products and other therapies. From designing IND-enabling studies to delivering full CMC analytical and QC support, we join your R&D team as a true partner. EAG scientists take time to understand both your commercial goals and the unique characteristics of your compound. We provide expert guidance to balance regulatory expectations with expediency and cost, and approach technical challenges with flexibility and resolve.

Materials Testing & Analysis

When it comes to understanding the physical structure, chemical properties and composition of materials, no scientific services company offers the breadth of experience, diversity…

When it comes to understanding the physical structure, chemical properties and composition of materials, no scientific services company offers the breadth of experience, diversity of analytical techniques of technical ingenuity of EAG. From polymers to composites, thin films to superalloys—we know how to leverage materials sciences to gain a competitive edge. At EAG, we don’t just perform testing, we drive commercial success—through thoughtfully designed investigations, technically superior analyses and expert interpretation of data.

Environmental Testing & Regulatory Compliance

Having helped develop the test methods that shape current regulatory guidelines, EAG chemists, biologists and toxicologists have evaluated the environmental impact of thousands of…

Having helped develop the test methods that shape current regulatory guidelines, EAG chemists, biologists and toxicologists have evaluated the environmental impact of thousands of active ingredients and formulations—from pesticides and pharmaceuticals to industrial chemicals and consumer products. Whether you are exploring “what if” scenarios, registering a new active ingredient or formulation, responding to a data call-in or seeking to understand the latest guidance, turn to EAG for technical excellence, sound advice, GLP-compliant study execution and expert interpretation.

Microelectronics Test & Engineering

Whether connecting the internet of things, guiding surgical lasers or powering the latest smart phone, integrated circuits and microelectronics touch nearly every aspect of human…

Whether connecting the internet of things, guiding surgical lasers or powering the latest smart phone, integrated circuits and microelectronics touch nearly every aspect of human life. In the world of technology, innovation and continuous improvement are imperatives—and being able to quickly and reliably test, debug, diagnose failures and take corrective action can make the difference between a doomed product launch and building a successful global brand. EAG offers you the world’s largest and most diverse collection of specialized analytical instrumentation, capacity to perform a variety of microelectronic tests in parallel, and the multi-disciplinary expertise required to draw true insight from data.

Custom Synthesis & Radiolabeling

No contract service provider has more experience performing custom synthesis and producing isotopically labeled compounds to support product development in life science, chemical…

No contract service provider has more experience performing custom synthesis and producing isotopically labeled compounds to support product development in life science, chemical and related industries than we do. From 14C and 3H radiolabeled clinical trial materials synthesized under cGMP, to stable-labeled active ingredients for metabolism and environmental fate/effects testing, turn to EAG. We have extensive experience with multi-step and other complex synthesis projects, and our comprehensive, in-house analytical services ensure quick turnaround of purity and structural confirmation.

Crop Biotechnology & Development

EAG combines biotechnology and protein characterization expertise with more than 50 years' experience analyzing chemical compounds in plant and environmental matrices to address…

EAG combines biotechnology and protein characterization expertise with more than 50 years’ experience analyzing chemical compounds in plant and environmental matrices to address the growing needs of the biotechnology crop industry. We offer a wide range of techniques required to fully characterize the event insertion and expressed proteins, as well as the various studies required to confirm the food, feed and environmental safety of products that represent the trait. From early-stage protein confirmation to GLP-compliant EDSP and allergenicity testing, we help you make faster, more informed development decisions and comply with evolving global regulations of genetically engineered crops.

Litigation Support & Expert Testimony

When you need solid science and investigative engineering to address product failures, inform legal strategy, protect intellectual property or address product liability disputes,…

When you need solid science and investigative engineering to address product failures, inform legal strategy, protect intellectual property or address product liability disputes, turn to EAG. We’ve provided technical consulting, analysis and expert testimony for hundreds of cases involving the aerospace, transportation, medical device, electronics, industrial and consumer product industries. Our team of experts understands the legal process and your need for responsiveness, effective communication, scientifically defensible opinion and confidentiality. From professional consulting to data review to trial preparation and expert witness testimony, ask EAG.

Techniques

Chromatography

Using an array of advanced separation techniques and innovative technology, we conduct highly precise analytical chromatography for various industries. Whether you want a closer…

Using an array of advanced separation techniques and innovative technology, we conduct highly precise analytical chromatography for various industries. Whether you want a closer look at the purity of your pharmaceutical or need to better understand an agrochemical’s components, EAG has the expertise to separate and evaluate any compound.

Mass Spectrometry

Need to evaluate the molecular structure of a compound or identify its origins? EAG knows how. With state-of-the-art tools, we can separate, vaporize and ionize the atoms and…

Need to evaluate the molecular structure of a compound or identify its origins? EAG knows how. With state-of-the-art tools, we can separate, vaporize and ionize the atoms and molecules in almost any pure or complex material to detect and obtain mass spectra of the components. We rely on decades of experience in mass spectrometry to provide our clients with precise analyses and the best detection limits.

Imaging

EAG is a world leader in high-resolution imaging down to the atomic level. We offer unmatched analytical know-how, generating extremely detailed surface and near surface images…

EAG is a world leader in high-resolution imaging down to the atomic level. We offer unmatched analytical know-how, generating extremely detailed surface and near surface images for various industries, from consumer electronics to nanotechnology. Using state-of-the-art equipment and innovative techniques, we conduct expert imaging to aid in failure analysis, dimensional analysis, process characterization, particle identification and more. If you want to investigate a material with angstrom scale resolution, you can count on EAG to get the job done quickly and precisely.

Spectroscopy

EAG offers a vast array of spectroscopic techniques to clients in various industries, from defense contractors to technology pioneers. We combine unparalleled expertise and…

EAG offers a vast array of spectroscopic techniques to clients in various industries, from defense contractors to technology pioneers. We combine unparalleled expertise and methodology with cutting-edge technology to analyze your organic, inorganic, metallic and composite materials for identification, compositional, structural and contaminant information. Whether you need expert spectroscopic analysis to improve your production process or to surmount a technical challenge, EAG is up to the task.

Physical/Chemical Characterization

Need to identify your unique material? Want to analyze the thermal properties of a sample, or measure the success of a process step? If it has to be done quickly and it has to be…

Need to identify your unique material? Want to analyze the thermal properties of a sample, or measure the success of a process step? If it has to be done quickly and it has to be done right, you can count on EAG. We offer a range of adaptable techniques and innovative methods to evaluate the physical and chemical characteristics of any compound. Our highly precise testing and analytical services will improve your production process, expedite R&D and help you conquer any technical challenge.

About

A Global Scientific Services Company

One of the most respected names in contract research and testing, EAG Laboratories is a global scientific services company operating at the intersection of science, technology and…

One of the most respected names in contract research and testing, EAG Laboratories is a global scientific services company operating at the intersection of science, technology and business. The scientists and engineers of EAG apply multi-disciplinary expertise, advanced analytical techniques and “we know how” resolve to answer complex questions that drive commerce around the world.

Our Customers

Science and technology transcend industry boundaries, and so does demand for EAG’s expertise. We partner with companies across a broad spectrum of high-tech, high-impact and…

Science and technology transcend industry boundaries, and so does demand for EAG’s expertise. We partner with companies across a broad spectrum of high-tech, high-impact and highly regulated industries. We help our customers innovate new and improved products, investigate manufacturing problems, perform advanced analyses to determine safety, efficacy and regulatory compliance, and protect their brands.

Our Company Culture

EAG’s corporate culture is firmly rooted in four guiding principles: “foster a growth mindset,” “find a better way,” “earn more loyal customers,” and “win…

EAG’s corporate culture is firmly rooted in four guiding principles: “foster a growth mindset,” “find a better way,” “earn more loyal customers,” and “win together.” Across all of our 20+ locations, you will find a true passion for science and the power of science to improve the world we live in. Hear what some of our ~1200 scientists, engineers and support personnel say about what it means to be part of EAG Laboratories.

Careers

EAG is growing, and we are always looking for talented, problem-solving oriented individuals to join our company. If you have a “we know how” spirit, we want to hear from you.…

EAG is growing, and we are always looking for talented, problem-solving oriented individuals to join our company. If you have a “we know how” spirit, we want to hear from you. Browse current openings now, and re-visit our careers page often.

How do you make better development decisions faster?

Comparison of Detection Techniques for Distribution of 14C Residues by HPLC

APPLICATION NOTE

Ki Chang Ahn, Jason LaMar, Thomas Fleischmann, and David Dohn

INTRODUCTION

Conduct of environmental fate, plant and animal metabolism studies with a 14C radiolabeled test substance includes chromatographic analysis of extracts to elucidate the distribution of transformation products (metabolites) in a test system. The distribution of metabolites in extracts are commonly determined using chromatographic separation with HPLC.

Detection of radiolabeled components from a HPLC separation is performed either by collection of fractions followed by liquid scintillation counting of collected fractions, or using a flow through radioisotope monitor. Traditional liquid scintillation counters (LSC) detect one sample at a time. Introduction of microplate LSC (TopCount, MicroBeta) increased the throughput of detection to 12-samples simultaneously.

METHODS

General Procedure for 14C Residue Detection

1. 14C-Treated animal or plant samples were extracted with an acetonitrile/water mixture and acetonitrile. Extracts were combined and concentrated prior to HPLC analysis.

2. HPLC separation of extracts were performed using a HPLC system equipped with a reverse phase column eluting with an aqueous to acetonitrile linear gradient.

3. Radioisotope flow monitor was a Beta-RAM (LabLogic) equipped with a 500 μL flow cell and auxiliary pump to mix scintillation cocktail with HPLC column eluent in a ratio of 3:1.

4. LSC was performed using Beckman LS 6000 or LS 6500 instruments or Perkin Elmer MicroBeta2 counter with 12-detectors. Counting time was 1 min per fractionated sample.

Instruments for 14C Radioactive Detection after Chromatographic Separation

A. Fractionation @ 30-sec. interval/Liquid Scintillation Counter: Beckman LS 6500 or LS 6000 IC liquid scintillation spectrophotometer.

B. Flow-through Radio-HPLC Detection using a Beta-RAM by LabLogic with 500 μL liquid cell.

C. Fractionation @ 15~20-sec. interval/MicroBeta Microplate Counter: PerkinElmer 2450 MicroBeta Microplate Counter including 12 detectors – (12 wells in a 96-well plate are counted simultaneously).

D. Fractionation to vials and microplates: Gilson FC204 Fraction Collector.

Instruments for [14C]Radioactive Detection after Chromatographic Separation

RESULTS

In Case of Analysis of Low Sample Matrix: Similar Chromatographic Resolution

A. HPLC/Typical fractionation (30-sec)/LSC: - 16,300 dpm injected - 100 μL injected
A. HPLC/Typical fractionation (30-sec)/LSC:

– 16,300 dpm injected

– 100 μL injected

B. HPLC/Flow-through radio detection beta RAM: - 16,300 dpm injected - 100 μL injected
B. HPLC/Flow-through radio detection beta RAM:

– 16,300 dpm injected

– 100 μL injected

C. HPLC/96-well fractionation (15-sec)/ MicroBeta - 16,300 dpm - 100 μL injected
C. HPLC/96-well fractionation (15-sec)/ MicroBeta

– 16,300 dpm

– 100 μL injected

In Case of Analysis of Severe Sample Matrix: Enhanced Chromatographic Resolution

B. HPLC/Flow-throchromatographic ugh radio detection beta- RAM: Poor resolution - 15,600 dpm injected - 500 μL injected
B. HPLC/Flow-throchromatographic ugh radio detection beta-RAM: Poor resolution

– 15,600 dpm injected

– 500 μL injected

C. HPLC/96-well fractionation (15-sec)/Microbeta2 counting: Enhanced chromatographic resolution, compared to B. - 3,000 dpm - 200 μL injected
C. HPLC/96-well fractionation (15-sec)/Microbeta2 counting: Enhanced chromatographic resolution, compared to B.

– 3,000 dpm

– 200 μL injected

Comparison in terms of sensitivity, speed, cost, and chromatographic resolution

Comparison in terms of sensitivity, speed, cost, and chromatographic resolution pt.1
Comparison in terms of sensitivity, speed, cost, and chromatographic resolution pt.2

 CONCLUSIONS

1. Sensitivity: MicroBeta counter > LSC >> Flow-through beta-RAM

2. Speed:

– For single sample

Flow-through beta-RAM > MicroBeta counter >> LSC

– For multiple samples

Flow-through beta-RAM > MicroBeta counter >> LSC

3. HPLC Chromatographic Resolution:

– For low sample matrix analysis

Flow-through beta-RAM = MicroBeta counter = LSC

– For high sample matrix analysis

MicroBeta counter >> LSC > Flow-through beta-RAM

4. Cost Effective:

Flow-through beta-RAM > MicroBeta counter >> LSC

5. Radioactive Waste Volume:

MicroBeta counter > Flow-through beta-RAM >> LSC

  • HPLC resolution using the traditional technique of fraction collection and liquid scintillation counter (LSC) equipped with a single detector is a function of fraction collection time (faster collection times results in higher resolution). The sensitivity by fraction collection is a function of counting time (longer counting time results in higher sensitivity). However, LSC of collected fractions is labor intensive and time consuming.
  • HPLC equipped with a radioisotope flow-through detector has the benefit of providing real time monitoring of the radioactive components. Resolution of chromatographic peaks and sensitivity in radioisotope flow monitors are functions of the flow cell volume, flow rate through the counting cell and counting efficiency (liquid cell or solid cell). Detection using radioisotope flow monitors often requires the researcher to compromise between speed of analysis with lower sensitivity than the traditional fraction collection/LSC technique.
  • Introduction of LSC instrument with capabilities to count 96-well plates with multiple detectors provides researchers improvements in counting time over the traditional fraction collection technique while maintaining high sensitivity.

 ACKNOWLEDGEMENTS

We thank Jeff Davis and Julie Huang for their kind contributions to this study.