Industries
Services

Pharmaceutical & Biopharmaceutical Development Services

EAG brings unparalleled expertise to the development and commercialization of small molecule drugs, biopharmaceuticals, antibody-drug conjugates (ADCs), drug-device combination…

EAG brings unparalleled expertise to the development and commercialization of small molecule drugs, biopharmaceuticals, antibody-drug conjugates (ADCs), drug-device combination products and other therapies. From designing IND-enabling studies to delivering full CMC analytical and QC support, we join your R&D team as a true partner. EAG scientists take time to understand both your commercial goals and the unique characteristics of your compound. We provide expert guidance to balance regulatory expectations with expediency and cost, and approach technical challenges with flexibility and resolve.

Materials Testing & Analysis

When it comes to understanding the physical structure, chemical properties and composition of materials, no scientific services company offers the breadth of experience, diversity…

When it comes to understanding the physical structure, chemical properties and composition of materials, no scientific services company offers the breadth of experience, diversity of analytical techniques of technical ingenuity of EAG. From polymers to composites, thin films to superalloys—we know how to leverage materials sciences to gain a competitive edge. At EAG, we don’t just perform testing, we drive commercial success—through thoughtfully designed investigations, technically superior analyses and expert interpretation of data.

Environmental Testing & Regulatory Compliance

Having helped develop the test methods that shape current regulatory guidelines, EAG chemists, biologists and toxicologists have evaluated the environmental impact of thousands of…

Having helped develop the test methods that shape current regulatory guidelines, EAG chemists, biologists and toxicologists have evaluated the environmental impact of thousands of active ingredients and formulations—from pesticides and pharmaceuticals to industrial chemicals and consumer products. Whether you are exploring “what if” scenarios, registering a new active ingredient or formulation, responding to a data call-in or seeking to understand the latest guidance, turn to EAG for technical excellence, sound advice, GLP-compliant study execution and expert interpretation.

Microelectronics Test & Engineering

Whether connecting the internet of things, guiding surgical lasers or powering the latest smart phone, integrated circuits and microelectronics touch nearly every aspect of human…

Whether connecting the internet of things, guiding surgical lasers or powering the latest smart phone, integrated circuits and microelectronics touch nearly every aspect of human life. In the world of technology, innovation and continuous improvement are imperatives—and being able to quickly and reliably test, debug, diagnose failures and take corrective action can make the difference between a doomed product launch and building a successful global brand. EAG offers you the world’s largest and most diverse collection of specialized analytical instrumentation, capacity to perform a variety of microelectronic tests in parallel, and the multi-disciplinary expertise required to draw true insight from data.

Custom Synthesis & Radiolabeling

No contract service provider has more experience performing custom synthesis and producing isotopically labeled compounds to support product development in life science, chemical…

No contract service provider has more experience performing custom synthesis and producing isotopically labeled compounds to support product development in life science, chemical and related industries than we do. From 14C and 3H radiolabeled clinical trial materials synthesized under cGMP, to stable-labeled active ingredients for metabolism and environmental fate/effects testing, turn to EAG. We have extensive experience with multi-step and other complex synthesis projects, and our comprehensive, in-house analytical services ensure quick turnaround of purity and structural confirmation.

Crop Biotechnology & Development

EAG combines biotechnology and protein characterization expertise with more than 50 years' experience analyzing chemical compounds in plant and environmental matrices to address…

EAG combines biotechnology and protein characterization expertise with more than 50 years’ experience analyzing chemical compounds in plant and environmental matrices to address the growing needs of the biotechnology crop industry. We offer a wide range of techniques required to fully characterize the event insertion and expressed proteins, as well as the various studies required to confirm the food, feed and environmental safety of products that represent the trait. From early-stage protein confirmation to GLP-compliant EDSP and allergenicity testing, we help you make faster, more informed development decisions and comply with evolving global regulations of genetically engineered crops.

Litigation Support & Expert Testimony

When you need solid science and investigative engineering to address product failures, inform legal strategy, protect intellectual property or address product liability disputes,…

When you need solid science and investigative engineering to address product failures, inform legal strategy, protect intellectual property or address product liability disputes, turn to EAG. We’ve provided technical consulting, analysis and expert testimony for hundreds of cases involving the aerospace, transportation, medical device, electronics, industrial and consumer product industries. Our team of experts understands the legal process and your need for responsiveness, effective communication, scientifically defensible opinion and confidentiality. From professional consulting to data review to trial preparation and expert witness testimony, ask EAG.

Techniques

Chromatography

Using an array of advanced separation techniques and innovative technology, we conduct highly precise analytical chromatography for various industries. Whether you want a closer…

Using an array of advanced separation techniques and innovative technology, we conduct highly precise analytical chromatography for various industries. Whether you want a closer look at the purity of your pharmaceutical or need to better understand an agrochemical’s components, EAG has the expertise to separate and evaluate any compound.

Mass Spectrometry

Need to evaluate the molecular structure of a compound or identify its origins? EAG knows how. With state-of-the-art tools, we can separate, vaporize and ionize the atoms and…

Need to evaluate the molecular structure of a compound or identify its origins? EAG knows how. With state-of-the-art tools, we can separate, vaporize and ionize the atoms and molecules in almost any pure or complex material to detect and obtain mass spectra of the components. We rely on decades of experience in mass spectrometry to provide our clients with precise analyses and the best detection limits.

Imaging

EAG is a world leader in high-resolution imaging down to the atomic level. We offer unmatched analytical know-how, generating extremely detailed surface and near surface images…

EAG is a world leader in high-resolution imaging down to the atomic level. We offer unmatched analytical know-how, generating extremely detailed surface and near surface images for various industries, from consumer electronics to nanotechnology. Using state-of-the-art equipment and innovative techniques, we conduct expert imaging to aid in failure analysis, dimensional analysis, process characterization, particle identification and more. If you want to investigate a material with angstrom scale resolution, you can count on EAG to get the job done quickly and precisely.

Spectroscopy

EAG offers a vast array of spectroscopic techniques to clients in various industries, from defense contractors to technology pioneers. We combine unparalleled expertise and…

EAG offers a vast array of spectroscopic techniques to clients in various industries, from defense contractors to technology pioneers. We combine unparalleled expertise and methodology with cutting-edge technology to analyze your organic, inorganic, metallic and composite materials for identification, compositional, structural and contaminant information. Whether you need expert spectroscopic analysis to improve your production process or to surmount a technical challenge, EAG is up to the task.

Physical/Chemical Characterization

Need to identify your unique material? Want to analyze the thermal properties of a sample, or measure the success of a process step? If it has to be done quickly and it has to be…

Need to identify your unique material? Want to analyze the thermal properties of a sample, or measure the success of a process step? If it has to be done quickly and it has to be done right, you can count on EAG. We offer a range of adaptable techniques and innovative methods to evaluate the physical and chemical characteristics of any compound. Our highly precise testing and analytical services will improve your production process, expedite R&D and help you conquer any technical challenge.

About

A Global Scientific Services Company

One of the most respected names in contract research and testing, EAG Laboratories is a global scientific services company operating at the intersection of science, technology and…

One of the most respected names in contract research and testing, EAG Laboratories is a global scientific services company operating at the intersection of science, technology and business. The scientists and engineers of EAG apply multi-disciplinary expertise, advanced analytical techniques and “we know how” resolve to answer complex questions that drive commerce around the world.

Our Customers

Science and technology transcend industry boundaries, and so does demand for EAG’s expertise. We partner with companies across a broad spectrum of high-tech, high-impact and…

Science and technology transcend industry boundaries, and so does demand for EAG’s expertise. We partner with companies across a broad spectrum of high-tech, high-impact and highly regulated industries. We help our customers innovate new and improved products, investigate manufacturing problems, perform advanced analyses to determine safety, efficacy and regulatory compliance, and protect their brands.

Our Company Culture

EAG’s corporate culture is firmly rooted in four guiding principles: “foster a growth mindset,” “find a better way,” “earn more loyal customers,” and “win…

EAG’s corporate culture is firmly rooted in four guiding principles: “foster a growth mindset,” “find a better way,” “earn more loyal customers,” and “win together.” Across all of our 20+ locations, you will find a true passion for science and the power of science to improve the world we live in. Hear what some of our ~1200 scientists, engineers and support personnel say about what it means to be part of EAG Laboratories.

Careers

EAG is growing, and we are always looking for talented, problem-solving oriented individuals to join our company. If you have a “we know how” spirit, we want to hear from you.…

EAG is growing, and we are always looking for talented, problem-solving oriented individuals to join our company. If you have a “we know how” spirit, we want to hear from you. Browse current openings now, and re-visit our careers page often.

How do you make continuous improvement profitable?

PYROLYSIS GAS CHROMATOGRAPHY/MASS SPECTROMETRY (PYRO-GC-MS)

Pyrolysis Gas Chromatography/Mass Spectrometry (Pyrolysis-GC-MS) is an analytical tool used to characterize a wide variety of polymers and composite materials that cannot be analyzed using traditional GC-MS The fundamental concept of Pyrolysis-GC-MS is not complicated but highly sensitive.  A sample is introduced to a heated environment and is broken down into smaller stable fragments/components (aka pyrolyzates) for analysis.  The pyrolyzates are then chromatographically separated in the same manner as traditional GC-MS.  Similar to traditional GC-MS, the fragments are separated utilizing a compound’s intrinsic affinity for a “stationary phase” (solid support with specialized coating).  Once the pyrolyzates are separated, the data is analyzed by highly skilled scientists who can deconvolute and interpret the data and subsequently provide useful information and insight on the original material.

In order to conduct a thorough investigation, the Pyrolysis temperature program must be set up in a very specific way.  When a sample is introduced into the Pyrolysis unit, it is instantly heated from ambient to the desired temperature in an inert atmosphere.  This rapid heating provides reproducible results. If additional tests are needed, the test sample is cooled in an inert atmosphere to room temperature and the Pyrolysis unit is reprogrammed. Several different experiments can be performed and they are explained below.

Types of Pyrolysis-GC-MS analyses:

  • A Single Shot Pyrolysis-GC-MS: The single shot is run at a single temperature, normally >500°C (dependent on the material being examined). The temperature of the sample is rapidly increased at a temperature rate <20msec to break bonds.  This is done in order to characterize the original sample.
  • A Double Shot Pyrolysis: This is normally run at both low (80-350°C) and high temperatures (500-800°C). The lower temperature analysis is known as a thermal desorption step.  This step can be used to examine low molecular weight components (monomers, oligomers, etc.), additives and residual solvents.  The higher temperatures allow for the total fragmentation of the higher molecular weight items such as the crosslinked material or the entire polymer backbone.
  • Evolved Gas Analysis (EGA): This is run by dropping the sample into the Pyrolysis unit set at a low temperature. The furnace is then increased to a high temperature at a set ramp rate.  The components that are off-gassed/evolved from the sample are then examined.  This testing is done in order to identify the temperature range for the desired component.  The Pyrolysis-GC-MS can then be set up the desired temperature range for a more in-depth study of the identified compound.  As a side note, Thermal Gravimetric Analysis (TGA) can also be used to aid in the determination of the temperature analysis profile for Pyrolysis-GC-MS.
  • Low Temperature (normally considered <350°C) Thermal Desorption:. This testing would be done for products where additives, residual solvents or other off-gassed components are desired but the polymer identification is not needed.
  • Reactive Pyrolysis-GC-MS: There are certain classes of compounds, for example polyesters, that have complex Pyrolysis mixtures (i.e. many degradation products, etc.).  These complex mixtures make the interpretation of the data difficult, if not impossible.  When a derivatization technique is utilized, a chemical reaction occurs while the sample is in the Pyrolysis chamber, allowing for a much easier interpretation of the derivatized products.
  • Heart Cut-EGA GC-MS (HC-EGA-GC-MS): This method is useful when looking for specific components in a highly complex matrix or when the full composition of a complex system is desired.  Heart-Cut Pyrolysis involves using the EGA technique to  isolate the desired elution times (i.e. zones).  The instrument is then programmed to isolate each specified zone identified by EGA.  Each zone is then individually analyzed.  This technique allows for the examination of regions that might contain multiple components under one peak but are not obvious during the regular Pyrolysis GC-MS analysis.  While extremely useful, this technique can add to the complexity and amount of time for the analysis.
  • Quantitative Pyrolysis-GC-MS: This would be done on a component that the amount present could not be determined using another technique.  An example component would be a low ppm additive (such as an Irganox, Irgafos, etc.), FAME components in rubbers, phthalates plus many other compounds.
  • Cryotrapping capabilities are available for the Pyrolysis GC-MS. Prior to introduction into the GC-MS, the pyrolyzates are cryotrapped using liquid nitrogen.  This is done to narrow the chromatographic band and improve the detection limit.

Pyrolysis-GC-MS virtually eliminates the need for the various sample preparation techniques required by traditional GC-MS (extraction, dilutions, concentrating the sample, various chemical reactions, heating/cooling, etc. are unnecessary).  The samples can be analyzed in an “as received” state unless they fall into the category of needing reactive Pyrolysis-GC-MS.

EAG Laboratories offers Pyrolysis-GC-MS services at our St. Louis, Missouri location, 1-800-659-7659.

Ideal Uses
  • Polymer Identification
  • Additive Identification
  • Qualitative and Quantitative Analysis of Components
  • Materials that are not suitable for Direct GC-MS
Technical Specification
  • Detection limit is determined by the component being analyzed – most components seen in the ppm range.
  • Temperature range is 30°C to 800°C.
  • Ramp rates can vary from 1°C/minute to 100°C/minute.
  • Nitrogen purged environment.
Strengths
  • Can examine materials and compounds that are not suitable for traditional GC-MS.
  • Able to study polymeric structures from pure systems to multiple block polymers.
  • Micrograms of samples are used for analysis.
  • Reduced sample preparation.
Limitations
  • Non-Homogenous samples might have variable results.
  • Does not detect most inorganic components.
  • Pyrolysis-GC-MS is a destructive technique.