UHPLC, Ultra-High-Performance Liquid Chromatography is similar to HPLC, in that it is a technique used to separate different constituents of a compound. Used predominately to identify, quantify and separate components of a mixture by using high pressure to push solvents through the column. In UHPLC, particle sizes less than 2um can be used, providing better separation than HPLC where particle size is limited to 5um. These smaller particles require higher pump pressures (100MPa vs.40 MPa, making this technique very efficient with fast analysis and higher resolution.