Cryo-TEM involves performing Transmission Electron Microscopy TEM analysis while keeping the sample at cryogenic temperatures, i.e. -170°C (or 103 K).
Transmission Electron Microscopy (TEM) is a technique that images a sample using an electron beam. High energy electrons (80-200 keV) are transmitted through electron transparent samples (~100 nm thick) and imaged on a plane.
The main reasons for the use of low temperature are
The production of thin (~100 nm) slices of frozen aqueous dispersions (‘vitrified ice layers’) is achieved using a Vitrobot ™ sample preparation tool, a dedicated cryo-sample-transfer unit and a cryo-TEM sample holder.
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