Ultraviolet–Visible Spectroscopy (UV-Vis)

UV-Vis Spectroscopy is used to determine analyte concentration either at one time or often over a desired time period.  The technique measures the absorption of light across the ultraviolet and visible light wavelengths through a liquid sample.  Samples are dispensed into cuvette and placed in the path between a UV-Vis light and a detector.  According to Beer-Lambert’s law, with a constant light path length and known absorption coefficient  (dependent upon wavelength),  the concentration of a compound in question can be determined from the light absorbed by the sample at that wavelength.