Ultraviolet–Visible Spectroscopy (UV-Vis)

UV-Vis Spectroscopy is used to determine analyte concentration either at one time or often over a desired time period.  The technique measures the absorption of light across the ultraviolet and visible light wavelengths through a liquid sample.  Samples are dispensed into cuvette and placed in the path between a UV-Vis light and a detector.  According to Beer-Lambert’s law, with a constant light path length and known absorption coefficient  (dependent upon wavelength),  the concentration of a compound in question can be determined from the light absorbed by the sample at that wavelength.

Ideal Uses
  • Quantitation of analytes
  • Quality assurance and control
  • Maximum wavelength determination
Technical Specifications

Wavelength range 190-1100 nm

Strengths
  • Fast sample analysis
  • Suitable for a wide variety of analytes
  • Much simpler than chromatographic techniques
  • User-friendly interface
  • Little maintenance required
Limitations
  • Subject to fluctuations from stray light and temperature changes
  • Relatively low sensitivity
  • Other sample components may cause interferences
  • Not as specific as chromatography
  • Requires a relatively large sample volume, > 0.2 mL

To enable certain features and improve your experience with us, this site stores cookies on your computer. Please click Continue to provide your authorization and permanently remove this message.

To find out more, please see our privacy policy.