UV-Vis Spectroscopy is used to determine analyte concentration either at one time or often over a desired time period. The technique measures the absorption of light across the ultraviolet and visible light wavelengths through a liquid sample. Samples are dispensed into cuvette and placed in the path between a UV-Vis light and a detector. According to Beer-Lambert’s law, with a constant light path length and known absorption coefficient (dependent upon wavelength), the concentration of a compound in question can be determined from the light absorbed by the sample at that wavelength.
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