Screening Multiple Analytes of the Same Chemical Family: HPLC and Organic Acids

Organic acids serve a variety of functions in food such as preservation, flavor, and nutrition. Some organic acids are naturally present in fruit and offer many health benefits. To evaluate organic acid content across fruit juices, the proper analytical tools for product investigation are necessary. Eurofins EAG Laboratories can identify and quantify the different organic acids using High Performance Liquid Chromatography (HPLC). This note demonstrates the abilities that Eurofins EAG has to quickly and accurately screen for common organic acids in fruit juices.

In this study, the objective was to screen four types of fruit juices (tomato, apple, orange, and coconut) for organic acids using high performance liquid chromatography with diode array detection (HPLC-DAD). A summary of test samples is presented in Table 1. 

This technique can utilize a variety of mobile phases, columns, and detectors to separate and identify specific components in simple to complex soluble sample matrices. The diode array detector is used to measure the sample’s absorption of light at different wavelengths to identify a specific analyte.



For analysis, the samples were diluted in water and analyzed against organic acid reference standards. The standards include oxalic acid, tartaric acid, malic acid, citric acid, succinic acid, and ascorbic acid. A summary of potential therapeutic applications from these selected organic acids are presented in Table 2.

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