Principles and Strengths of In Vitro Testing

In Vitro model systems can be specified to the test sample’s intended use. 2D cell cultures are grown rapidly for high-throughput or multi-sample analysis with minimized variation between treatments. Cell cultures can also be made into 3D tissue models. These models contain differentiated cell layers to physiologically represent complex tissues and evaluate specific endpoints. Using specific geometries, in vitro cell culture models can be used to evaluate simple and complex biological responses.

To assess different biological endpoints, various forms of analytical methods may be implemented. Measurable responses of the in vitro models include cell death, growth inhibition, genetic alterations, changes in surface marker expression and altered metabolism.